Nikto support scanning multiple hosts in the same session via a text file of host names or IPs. Instead of giving a host name or IP for the -h (-host) option, a file name can be given. A file of hosts must be formatted as one host per line, with the port number(s) at the end of each line. Ports can be separated from the host and other ports via a colon or a comma. If no port is specified, port 80 is assumed.

This is an example of a valid hosts file: